Genetic dissection of the mamAB and mms 6 operons reveals a gene set
نویسندگان
چکیده
Genetic dissection of the mamAB and mms6 operons reveals a gene set essential for 1 magnetosome biogenesis in Magnetospirillum gryphiswaldense 2 3 4 5 Anna Lohße, Sarah Borg, Oliver Raschdorf, Isabel Kolinko, Éva 6 Tompa, Mihály Pósfai, Damien Faivre, Jens Baumgartner, and Dirk 7 Schüler* 8 9 10 11 12 13 14 1 Ludwig-Maximilians-Universität München, Dept. Biologie I, Mikrobiologie, 15 LMU Biozentrum, Großhaderner Str. 4, D-82152 Planegg Martinsried, 16 Germany 17 2 University of Pannonia, Dept. of Earth and Environmental Sciences, Veszprém, 18 POB 158, H-8200, Hungary 19 3 Max-Planck-Institut für Kolloidund Grenzflächenforschung, Am Mühlenberg 1, 2
منابع مشابه
Transcriptional organization and regulation of magnetosome operons in Magnetospirillum gryphiswaldense.
Genes involved in magnetite biomineralization are clustered within the genomic magnetosome island of Magnetospirillum gryphiswaldense. Their transcriptional organization and regulation were studied by several approaches. Cotranscription of genes within the mamAB, mamDC, and mms clusters was demonstrated by reverse transcription-PCR (RT-PCR) of intergenic regions, indicating the presence of long...
متن کاملGenetic dissection of the mamAB and mms6 operons reveals a gene set essential for magnetosome biogenesis in Magnetospirillum gryphiswaldense.
Biosynthesis of bacterial magnetosomes, which are intracellular membrane-enclosed, nanosized magnetic crystals, is controlled by a set of >30 specific genes. In Magnetospirillum gryphiswaldense, these are clustered mostly within a large conserved genomic magnetosome island (MAI) comprising the mms6, mamGFDC, mamAB, and mamXY operons. Here, we demonstrate that the five previously uncharacterized...
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Bacterial magnetosomes are membrane-enveloped, nanometer-sized crystals of magnetite, which serve for magnetotactic navigation. All genes implicated in the synthesis of these organelles are located in a conserved genomic magnetosome island (MAI). We performed a comprehensive bioinformatic, proteomic and genetic analysis of the MAI in Magnetospirillum gryphiswaldense. By the construction of larg...
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Although membrane-bounded compartments are commonly considered a unique eukaryotic characteristic, many species of bacteria have organelles. Compartmentalization is well studied in eukaryotes; however, the molecular factors and processes leading to organelle formation in bacteria are poorly understood. We use the magnetosome compartments of magnetotactic bacteria as a model system to investigat...
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